Two-Dimensional NMR and Protein Structure
نویسندگان
چکیده
منابع مشابه
Two-Dimensional NMR Lineshape Analysis
NMR titration experiments are a rich source of structural, mechanistic, thermodynamic and kinetic information on biomolecular interactions, which can be extracted through the quantitative analysis of resonance lineshapes. However, applications of such analyses are frequently limited by peak overlap inherent to complex biomolecular systems. Moreover, systematic errors may arise due to the analys...
متن کاملSpectral editing of two-dimensional magic-angle-spinning solid-state NMR spectra for protein resonance assignment and structure determination.
Several techniques for spectral editing of 2D (13)C-(13)C correlation NMR of proteins are introduced. They greatly reduce the spectral overlap for five common amino acid types, thus simplifying spectral assignment and conformational analysis. The carboxyl (COO) signals of glutamate and aspartate are selected by suppressing the overlapping amide N-CO peaks through (13)C-(15)N dipolar dephasing. ...
متن کاملTwo-Dimensional Fourier Transformation in NMR
The idea of two-dimensional Fourier transformation was originated by Jeener (1) in 1971, but the very wide significance of his proposal does not seem to have been generally realized until several years later, when several elegant applications were described (2-6). The basic idea is very simple. In modern nmr spectrometers, spectra are normally obtained by Fourier transformation of a transient f...
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ژورنال
عنوان ژورنال: Annual Review of Biochemistry
سال: 1989
ISSN: 0066-4154,1545-4509
DOI: 10.1146/annurev.bi.58.070189.001255